Lesson #4: Two Photon Imaging

Two-photon calcium imaging is a powerful way to simultaneously image hundreds or even thousands of cells simultaneously. The Allen Brain Observatory contains two-photon imaging data grouped together by mouse cre line, brain structure, and imaging depth.

In this lesson, you’ll work directly with this data through the AllenSDK.

For an example of what this type of data looks like in action, run the cell below.

from IPython.display import YouTubeVideo

YouTubeVideo('vqGPp4d3LGw')

Learn More! Below you’ll find two lectures from the Allen Institute which discuss optical imaging and the kinds of experiments they conduct to understand visual perception in a mouse.

YouTubeVideo('4ZoKGFLg0HQ')

YouTubeVideo('m13Tk9_Ul-M')